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Reassembled Galactose oxidase

From the fungus Dactylium dendroides(n1_1GOF.pdb)

(Display produced using JSmol and HTML5. If you don't see a structure try a different browser.)

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View chain A down the beta-sheet barrel (aka Propeller Axis). Copper atoms white. Auxiliary domains are hidden
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Each chain consists of a catalytic domain and an auxiliary domain. I have labelled them as if separate chains. A, B, C, and D are catalytic domains and E, F, G, and H their respective auxiliary domains. As with other enzymes, the catalytic site is enclosed and physically constrained. Constraint is produced by the focussed compressive stress of four beta-sheet barrels, also known as "propellers", very similar to the structure of neuraminidases. The orientation of the barrels deviates from a regular tetrahedron to provide the optimum spatial distribution of stress for catalysis at the active site. In nature, this enzyme acts on the terminal residue of an oligosaccharide and therefore must provide a passageway through which the oligosaccharide can extend from the outside to the active site.

For further information about this protein, see the paper "Proposed tetrahedral quaternary structure for Galactose Oxidase"referred to in the posts of 30 September 2007 and 10 July 2011 on nativeproteins.blogspot.com

Or contact the author, Dr Don Vanselow at dvanselow@hotmail.com

Further details of JSmol available here